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µM to pmol/µl

PostPosted: Wed Nov 14, 2007 10:18 pm
by Guest
Just come across this rather helpful website and wondered if anyone was able to clear this up for me. It's with regards to primer stocks for pcr experiments.

I have my primers as 100µM and I need them at 3.2 pmol/µl - can anyone help? I'm still confused that mol and M are different which probably is the reason why I can't understand how you can have mol/µl.

Thanks in advance
R

Re: µM to pmol/µl

PostPosted: Thu Nov 15, 2007 5:39 am
by Guest
Anonymous wrote:Just come across this rather helpful website and wondered if anyone was able to clear this up for me. It's with regards to primer stocks for pcr experiments.

I have my primers as 100µM and I need them at 3.2 pmol/µl - can anyone help? I'm still confused that mol and M are different which probably is the reason why I can't understand how you can have mol/µl.

Thanks in advance
R


ALthough the term has been deprecated in modern SI usage, M has been used as a symol for molarity. 1 M is a solution of 1 mol/L, the preferred way to state it in modern SI. Therefore your 100 µM solution is 100 µmol/L, or 100 pmol/µL. It is roughly 31.25X too strong and needs to be diluted.

Re: µM to pmol/µl

PostPosted: Mon Feb 08, 2010 11:56 pm
by T22
100um/L
100um/ 1000ml
0.1um/ml
100nm/ml
0.1nm/ul
100pm/ul
You haven't given the final working solution volume e.g. I think say you wanted 75ul working solution
3.2% of 75=2.4ul primer stock
75-2.4=72.6 ul DH2O
I think that this is right, I am currently trying to do the same.
Actually it is right. Definitely.

Re: µM to pmol/µl

PostPosted: Wed May 30, 2012 4:51 pm
by SMILE
Hi i am very confused, my supervisor has asked me to convert primers of stock 100uM/ul to 100pM/ul which im not sure how to proceed as ive see pM/ul converted from uM/l... pls help... thanks

Re: µM to pmol/µl

PostPosted: Wed May 30, 2012 4:58 pm
by SMILE
Hi i am very confused, my supervisor has asked me to convert primers of stock 100uM/ul to 100pM/ul which im not sure how to proceed as ive see pM/ul converted from uM/l... pls help...i feel 100uM/ul is same as 100pM/ul ..pls correct me... thanks....

Re: µM to pmol/µl

PostPosted: Wed Dec 12, 2012 3:08 pm
by guest
I have a confusion in the dilution of my primer. the company has provided the data that if I resuspend the oligo in 200 microlitre of TE buffer then the concentration would be 223.71 (pm/microlitre). How do I convert it to 100 pm/microlitre? I would be highly obliged if you could help me.

Re: µM to pmol/µl

PostPosted: Wed Jan 02, 2013 11:08 am
by Tania
C1 x V1 = C2 x V2


223.71 (C1) * X(V1) = 100 (C2) * final working solution volume (V2)

X (V1)= 100 (C2) * final working solution volume (V2)
223.71 (C1)

Re: µM to pmol/µl

PostPosted: Wed Jan 16, 2013 1:07 am
by Me2
uM = pmol/uL. Therefore, 100 uM = 100 pmol/uL

Re: µM to pmol/µl

PostPosted: Fri Mar 08, 2013 10:27 am
by Parker88
I've asked that convert primers of stock 100uM/ul that he is not sure how to proceed.


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Re: µM to pmol/µl

PostPosted: Mon Jun 16, 2014 8:14 pm
by misla
can anyone pliz help me with this calculation.How would you dissolve 72nmol of primer to get 20uM stock?tq