My boss want micrograms from microliters

[desiuny]

How do i get 3 ug out of a 1mg/ml solution?

Everytime he says i need to put 3 ug or 2 ug or whatever, i don't know what to do. It's so frustrating.

I know that if the solution is 1ug/ml then 1 ug = 1uL

So how do i do it when the solution is 1mg/mL?

Please help me, i'm desperate, it's a new job and i don't want to sound ignorant but i forgot how to do it and i don't want him to think that i'm incompetent by asking him.

Thanks!

Des

[Guest]

How do i get 3 ug out of a 1mg/ml solution?

Everytime he says i need to put 3 ug or 2 ug or whatever, i don't know what to do. It's so frustrating.

I know that if the solution is 1ug/ml then 1 ug = 1uL


Des

Actually if it were 1 ug/mL, then 1 ug would equal 1 mL.

At the present concentration, you get 1 mg = 1000 ug per milliliter. If you only want 3 ug, you'd have to measure 0.003 mL, which I don't think is practical. You need to dilute your solution.

If you took 1 mL of the 1mg/mL solution and diluted it in enough distilled water to make 1 L, then it would be 1 mg/L or 1 ug/mL, and you'd need 3 mL to get 3 ug of substance.