µM to pmol/µl

[Guest]

Just come across this rather helpful website and wondered if anyone was able to clear this up for me. It's with regards to primer stocks for pcr experiments.

I have my primers as 100µM and I need them at 3.2 pmol/µl - can anyone help? I'm still confused that mol and M are different which probably is the reason why I can't understand how you can have mol/µl.

Thanks in advance
R

[Guest]

Just come across this rather helpful website and wondered if anyone was able to clear this up for me. It's with regards to primer stocks for pcr experiments.

I have my primers as 100µM and I need them at 3.2 pmol/µl - can anyone help? I'm still confused that mol and M are different which probably is the reason why I can't understand how you can have mol/µl.

Thanks in advance
R

ALthough the term has been deprecated in modern SI usage, M has been used as a symol for molarity. 1 M is a solution of 1 mol/L, the preferred way to state it in modern SI. Therefore your 100 µM solution is 100 µmol/L, or 100 pmol/µL. It is roughly 31.25X too strong and needs to be diluted.

[T22]

100um/L
100um/ 1000ml
0.1um/ml
100nm/ml
0.1nm/ul
100pm/ul
You haven't given the final working solution volume e.g. I think say you wanted 75ul working solution
3.2% of 75=2.4ul primer stock
75-2.4=72.6 ul DH2O
I think that this is right, I am currently trying to do the same.
Actually it is right. Definitely.

[SMILE]

Hi i am very confused, my supervisor has asked me to convert primers of stock 100uM/ul to 100pM/ul which im not sure how to proceed as ive see pM/ul converted from uM/l... pls help... thanks

[SMILE]

Hi i am very confused, my supervisor has asked me to convert primers of stock 100uM/ul to 100pM/ul which im not sure how to proceed as ive see pM/ul converted from uM/l... pls help...i feel 100uM/ul is same as 100pM/ul ..pls correct me... thanks....